全文获取类型
收费全文 | 450篇 |
免费 | 21篇 |
国内免费 | 1篇 |
专业分类
林业 | 18篇 |
农学 | 20篇 |
71篇 | |
综合类 | 19篇 |
农作物 | 28篇 |
水产渔业 | 71篇 |
畜牧兽医 | 183篇 |
园艺 | 2篇 |
植物保护 | 60篇 |
出版年
2022年 | 2篇 |
2021年 | 18篇 |
2020年 | 8篇 |
2019年 | 9篇 |
2018年 | 10篇 |
2017年 | 16篇 |
2016年 | 17篇 |
2015年 | 7篇 |
2014年 | 18篇 |
2013年 | 30篇 |
2012年 | 22篇 |
2011年 | 24篇 |
2010年 | 16篇 |
2009年 | 18篇 |
2008年 | 38篇 |
2007年 | 29篇 |
2006年 | 30篇 |
2005年 | 27篇 |
2004年 | 21篇 |
2003年 | 32篇 |
2002年 | 15篇 |
2001年 | 15篇 |
2000年 | 10篇 |
1999年 | 13篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 6篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1984年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有472条查询结果,搜索用时 109 毫秒
461.
中国中亚热带网纹红土的地球化学特征与沉积环境 总被引:3,自引:0,他引:3
130个样品的粒度、黏土矿物组成、地球化学特征以及Sr、Nd同位素分析表明,广泛分布在中亚热带地区并且具有一定厚度的第四纪网纹红土为加积型沉积。该类沉积粒度组成与北方风尘沉积类似,粉砂含量占优势,>50μm含量小于5%,5~50μm组分41.2%~61.5%,<5μm黏粒26.9%~55.8%;黏土矿物组成中含有较多的石英、云母和高岭石;全土体的平均硅铝率为7.96,硅铁铝率6.11;稀土元素丰度179.6 mg kg-1,与地壳平均值(178 mg kg-1)、北京郊区1998年12月大气粉尘稀土总量(178.0 mg kg-1)以及两块中国黄土标样(178.2mg kg-1)非常接近,并且表现为轻稀土强烈富集,重稀土淋失,Ce元素正异常;网纹红土全岩样品的87Sr/86Sr值界于0.725 657~0.727 714之间,Nd同位素0.511 989~0.512 098,与佳县上新世红黏土和黄土的酸不溶相性物质接近。理化特征指示,第四纪加积型网纹红土与北方黄土中的古土壤以及上新世红黏土的形成环境类似,并且具有稳定的物质来源。 相似文献
462.
463.
Current modernization of an irrigation system degrades fish habitats in quantity and quality and is a significant concern for biodiversity conservation and management of ecosystem services in rice farming landscapes. Irrigation systems consist of various types of channels, whose hydrological and hydraulic heterogeneities can contribute to the coexistence of diverse fish species in this flooded habitat. We compared seasonal patterns of fish species richness and abundance between main and branch channels which have different functions in an irrigation system with different flow temporality and magnitude: the main channels (mean width 425 cm and mean discharge 0.467 m3/s in irrigation period) and the branch channels (176 cm and 0.115 m3/s, respectively). The branch channels are small and temporary, but densities of fish species richness and abundance were not smaller than the main channels during the irrigation period. Further, clear positive hysteretic loops in species richness and abundance with discharge were found in the branch channels, which indicates that fish species richness and abundance gradually increased with discharge during this hydroperiod. Water velocity strongly constrained species richness and abundance in the main channels but not in the branch channels partially because of slow-flow patches at microhabitat scale with a larger vegetation coverage. We also found that the main channels provided deeper habitats in the non-irrigation period and contributed to maintain fish species richness and abundance. Therefore, managing both the main and branch channels as an indispensable, interconnected channel network can be a key for fish habitat conservation in an irrigation system. 相似文献
464.
Hideyuki Mannen Riku Yonesaka Aoi Noda Takeshi Shimogiri Ichiro Oshima Kiyomi Katahira Misao Kanemaki Tetsuo Kunieda Yousuke Inayoshi Fumio Mukai Shinji Sasazaki 《Animal Science Journal》2017,88(5):739-744
This study aims to estimate the mitochondrial genetic diversity and structure of Japanese Polled and Kuchinoshima feral cattle, which are maintained in small populations. We determined the mitochondrial DMA (mtDNA) displacement loop (D‐loop) sequences for both cattle populations and analyzed these in conjunction with previously published data from Northeast Asian cattle populations. Our findings showed that Japanese native cattle have a predominant, Asian‐specific mtDNA haplogroup T4 with high frequencies (0.43–0.81). This excluded Kuchinoshima cattle (32 animals), which had only one mtDNA haplotype belonging to the haplogroup T3. Japanese Polled showed relatively lower mtDNA diversity in the average sequence divergence (0.0020) than other Wagyu breeds (0.0036–0.0047). Japanese Polled have been maintained in a limited area of Yamaguchi, and the population size is now less than 200. Therefore, low mtDNA diversity in the Japanese Polled could be explained by the decreasing population size in the last three decades. We found low mtDNA diversity in both Japanese Polled and Kuchinoshima cattle. The genetic information obtained in this study will be useful for maintaining these populations and for understanding the origin of Japanese native cattle. 相似文献
465.
Genomic evaluation using SNP‐ and haplotype‐based genomic relationship matrices in a closed line of Duroc pigs 下载免费PDF全文
Yoshinobu Uemoto Shuji Sato Takashi Kikuchi Sachiko Egawa Kimiko Kohira Hironori Sakuma Satoshi Miyashita Shinji Arata Takatoshi Kojima Keiichi Suzuki 《Animal Science Journal》2017,88(10):1465-1474
A simulation analysis and real phenotype analysis were performed to evaluate the impact of three different relationship matrices on heritability estimation and prediction accuracy in a closed‐line breeding of Duroc pigs. The numerator relationship matrix (NRM), single nucleotide polymorphism (SNP)‐based genomic relationship matrix (GRM) (GS), and haplotype‐based GRM (GH) were applied in this study. We used PorcineSNP60 genotype array data (38 114 SNPs) of 831 Duroc pigs with four selection traits. In both heritability estimation and prediction accuracy, the accuracy depended on the number of animals with records. For heritability estimation, a large difference in the results among three relationship matrices was not shown, but the trend of the estimated heritabilities between GRMs, that is GS < GH, was shown in this population. For the accuracy of prediction values in test animals, the accuracies of prediction values obtained by two GRMs were higher than that by the NRM in this population. The accuracies obtained by GRMs using animals with no records were lower than that by the NRM using animals with their performance records, but were close to that by the NRM using animals with full‐sib testing records. 相似文献
466.
Voltage‐sensitive sodium channel mutations S989P + V1016G in Aedes aegypti confer variable resistance to pyrethroids,DDT and oxadiazines 下载免费PDF全文
BACKGROUND
Aedes aegypti is a vector of several important human pathogens. Control efforts rely primarily on pyrethroid insecticides for adult mosquito control, especially during disease outbreaks. A. aegypti has developed resistance nearly everywhere it occurs and insecticides are used. An important mechanism of resistance is due to mutations in the voltage‐sensitive sodium channel (Vssc) gene. Two mutations, in particular, S989P + V1016G, commonly occur together in parts of Asia.RESULTS
We have created a strain (KDR:ROCK) that contains the Vssc mutations S989P + V1016G as the only mechanism of pyrethroid resistance within the genetic background of Rockefeller (ROCK), a susceptible lab strain. We created KDR:ROCK by crossing the pyrethroid‐resistant strain Singapore with ROCK followed by four backcrosses with ROCK and Vssc S989P + V1016G genotype selections. We determined the levels of resistance conferred to 17 structurally diverse pyrethroids, the organochloride DDT, and oxadiazines (VSSC blockers) indoxacarb (proinsecticide) and DCJW (the active metabolite of indoxacarb). Levels of resistance to the pyrethroids were variable, ranging from 21‐ to 107‐fold, but no clear pattern between resistance and chemical structure was observed. Resistance is inherited as an incompletely recessive trait. KDR:ROCK had a > 2000‐fold resistance to DDT, 37.5‐fold cross‐resistance to indoxacarb and 13.4‐fold cross‐resistance to DCJW.CONCLUSION
Etofenprox (and DDT) should be avoided in areas where Vssc mutations S989P + V1016G exist at high frequencies. We found that pyrethroid structure cannot be used to predict the level of resistance conferred by kdr. These results provide useful information for resistance management and for better understanding pyrethroid interactions with VSSC. © 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献467.
Miloš Havelka He Zhou Seishi Hagihara Masaki Ichimura Takafumi Fujimoto Etsuro Yamaha Shinji Adachi Katsutoshi Arai 《Fisheries Science》2017,83(4):587-595
We investigated the source of spontaneous polyploidization in the critically endangered Acipenser mikadoi. Fourteen sib progeny of A. mikadoi and 11 hybrids between an A. mikadoi female and a Huso dauricus male, all showing atypically high ploidy, were analysed. Parent assignment based on five highly polymorphic microsatellite markers confirmed spontaneous duplication of maternal chromosome sets via retention of the second polar body to be the source of spontaneous polyploidization. To our knowledge, this provides the first evidence of the maternal origin of spontaneous polyploidization in A. mikadoi. Factorial correspondence analysis of the multilocus microsatellite genotypes placed the parent fish of spontaneous polyploids in clearly delineated clusters of A. mikadoi and H. dauricus, and parent fish had mitochondrial control region haplotypes corresponding to their presumed species. Thus, parent fish were confirmed to be of pure genetic origin, and hybridization did not promote the observed spontaneous polyploidization. 相似文献
468.
Kenta Adachi Hiroshi Miyake Takashi Kuramochi Kanta Mizusawa Sei-ichi Okumura 《Fisheries Science》2017,83(1):107-112
Cnidarians are considered ancestral metazoans and, therefore, are important taxa for studying animal evolution. However, little is known about the group’s genome size (C value), which is an important parameter in whole-genome sequencing. To address this issue, we measured the C values of 27 cnidarian species from Japan, using flow cytometry, and found that they ranged from 0.26 to 3.56 pg. Excluding the results for Agalma elegans and Physalia physalis (order Siphonophorae), which had the highest C values among the species included in the present study, the C values for the cnidarians were 0.26–1.49 pg. In particular, we found that hydrozoans possessed relatively large and wide-ranging C values, indicating that evolution within the group involved considerable gains or losses of genomic content. Overall, the C values reported in the present study could be valuable for whole-genome sequencing, using next-generation sequencers, and for future research in cytogenetics. 相似文献
469.
Satoshi KAMOTO Masahiro SHINADA Daiki KATO Masaya TSUBOI Sho YOSHIMOTO Ryohei YOSHITAKE Shotaro ETO Namiko IKEDA Yosuke TAKAHASHI Yuko HASHIMOTO James CHAMBERS Kazuyuki UCHIDA Shinji YAMADA Mika K. KANEKO Ryohei NISHIMURA Yukinari KATO Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1795
Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. Podoplanin is also expressed in cancer-associated fibroblasts and induces tumor malignancy. The objective of this study was to evaluate podoplanin expression in various types of feline tumor tissues. Immunohistochemical analysis revealed that podoplanin was expressed in cells of 13/15 (87%) squamous cell carcinomas and 5/19 (26%) fibrosarcomas. Moreover, cancer-associated fibroblasts expressed podoplanin in most tumor types, including 18/21 (86%) mammary adenocarcinoma tissues. Our findings demonstrate that various types of feline tumor tissues expressed podoplanin, indicating the importance of the comparative aspects of podoplanin expression, which may be used as a novel research model for podoplanin biology. 相似文献
470.
Fisheries Science - A part of fishery waste, such as scallop or oyster shells, are utilized for fertilizer, feed, soil conditioner, or fishing ground preparations, and most of the shells from... 相似文献